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Rev Clin Periodoncia Implantol Rehabil Oral. 2008;1:57-60
TRABAJOS DE INVESTIGACIÓN [PDF]

Recuento de Células Infiltrantes CD4+, CD8+ y CD19+ en el Tejido Gingival de Individuos con Periodontitis Crónica

Recount of CD4+, CD8+ and CD19+ Infiltrate Cells in Gingival Tissues of Subjects with Chronic Periodontitis

Cisterna C1, Rivera O1, Dutzan N1.

1Laboratorio de Biología Periodontal, Facultad de Odontología, Universidad de Chile. Chile.

Resumen

La periodontitis es una enfermedad inflamatoria del tejido periodontal, iniciada por el biofilm subgingival, que genera la destrucción progresiva de las estructuras de inserción dentaria. La naturaleza de la respuesta inflamatoria, determinaría el carácter destructivo de la enfermedad, considerándose un importante modelo para investigar el potencial patológico del infiltrado celular. En la periodontitis, este infiltrado incluye linfocitos T y B. Los linfocitos T CD4+ y T CD8+, efectores de la inmunidad celular, son claves en la inmunorregulación, pues pueden generar una respuesta celular o humoral. La relación T CD4+/CD8+ es considerada un indicador de la función del sistema inmune. Para determinar los porcentajes de células CD4+, CD8+ y CD19+ en el tejido gingival de individuos con periodontitis crónica y periodonto sano, y el valor de la razón entre las células CD4+/CD8+, tomamos biopsias de tejido gingival a 17 sujetos con periodontitis crónica y 11 periodontalmente sanos. Las muestras fueron digeridas en colagenasa, inmunotipificadas y analizadas mediante citometría de flujo, obteniendo que los porcentajes de todas las poblaciones estudiadas, así como, el valor de la razón CD4+/CD8+ aumentaron en las muestras de tejido enfermo respecto al tejido sano. Todas estas diferencias fueron estadísticamente significativas. Concluimos que las células CD4+, CD8+ y CD19+, son las más abundantes dentro del infiltrado celular de la periodontitis crónica, y suponemos que las células CD4+ serían claves en la regulación de la respuesta inmune de la enfermedad, dado que su población registró el mayor aumento en el tejido enfermo.

Palabras claves: Periodontitis crónica, linfocitos, infiltrado celular.

Abstract

The periodontitis is an inflammatory disease of the periodontal tissues, initiated by subgingival biofilm, that result in the progressive destruction of the structures that support the teeth. The nature of inflammatory response, could determine the destructive character of disease, and provides an important model for investigation of the pathological potential of cellular infiltrates. In the periodontitis, the infiltrates includes lymphocytes T and B. The lymphocytes T CD4+ and T CD8+, effectors of cellular immunity, are key cells in immunoregulation because they can lead to cellular or humoral response. The ratio of CD4+/CD8+ is considered to be an important indicator for immune system functions. For determinate the percentages of CD4+, CD8+ and CD19+ cells in the gingival tissues of periodontal and healthy subjects, and evaluate the CD4+/CD8+ cells ratio, were obtained biopsies of gingival tissues from 17 chronic periodontitis patients and 11 periodontally healthy subjects. The samples were incubated in collagenase, inmunolabeling and analyzed using flow cytometry. The obtained percentages in every studied cell population, as well, the ratio CD4+/CD8+ were increased in the periodontitis biopsies respect to healthy tissues. Every difference was statistically significant. We conclude that CD4+, CD8+ and CD19+, are the most abundant cells in the chronic periodontitis cellular infiltrate, and we suspect that CD4+ cells could be key on the immune response regulation to the disease, since this population recorded the higher increase in sick tissues.

Key words: Chronic periodontitis, lymphocytes, cellular infiltrates

El Texto completo solo está disponible en PDF

 

Bibliografía

1.
S. Socransky,A. Haffajee
The bacterial etiology of destructive periodontal disease: current concepts
J periodontol., 63 (1992), pp. 322-331
2.
S. Socransky,A. Haffajee,M. Cugini,C. Smith,Kent Jr.
Microbial complexes in subgingival plaque
J Clin Periodontol., 25 (1998), pp. 134-144
3.
K. Kornman,R. Page,M. Tonetti
The Host response to the microbial challenge in periodontitis: assembling the players
Periodontol, 14 (2000 1997), pp. 33-53
4.
R. Page,H. Schroeder
Pathogenesis of inflammatory periodontal disease. A summary of current work
Lab Invest., 34 (1976), pp. 235-249
5.
R. Vernal,N. Dutzan,M. Hernández
High expression levels of receptor activator of nuclear factor-kappa B ligand associated with human chronic periodontitis are mainly secreted by CD4+ T lymphocytes
J Periodontol., 77 (2006), pp. 1772-1780
6.
H. Takayanagi
Inflammatory bone destruction and osteoimmunology
J Periodontal Res., 40 (2005), pp. 287-293
7.
R. Orbak,K. Erciyas,H. Erzurum
Flow cytometric analysis of T-lymphocyte subsets after different treatment methods in smokers and non smokers with chronic periodontitis
Int Dent J., 53 (2003), pp. 159-164
8.
K. Erciyas,R. Orbak,F. Kavrut,T. Demir,I.I. Kaya
The changes in T lymphocyte subsets following periodontal treatment in patients with chronic periodontitis
J Periodont Res., 41 (2006), pp. 165-170
9.
K. Malberg,A. Molle,D. Streuer,P. Gangler
Determination of lymphocyte populations and subpopulations extracted from chronically inflamed human periodontal tissues
J Clin Periodontol., 19 (1992), pp. 155-158
10.
E.D. Stoufi,M.A. Taubman,J.L. Ebersole,D.J. Smith
Preparation and characterization of human gingival cells
J Periodontal Res., 22 (1987), pp. 144-149
11.
X. Han,T. Kawai,J. Eastcott,M. Taubman
Bacterial-responsive B lymphocytes induce periodontal bone resorption
The journal of immunology., 176 (2006), pp. 625-631
12.
Y. Teng
The role of acquired immunity and periodontal disease progression
Crit Rev Oral Biol Med., 14 (2003), pp. 237-252
13.
R.A. Reinhardt,R.W. Bolton,T. McDonald,L.M. DuBois,W.B. Kaldahl
In situ lymphocyte subpopulations from active versus stable periodontal sites
J Periodontol., 59 (1988), pp. 656-670
14.
B. Liljenberg,J. Lindhe,T. Berglundh,G. Dahlen,R. Jonsson
Some microbiological, histopathological and inmunohistochemical characteristics of progressive periodontal disease
J Clin Periodontol., 21 (1994), pp. 720-727
15.
G. Seymour,M. Taubman,J. Eastcott,E. Gemmell,D. Smith
CD29 expression on CD4+ gingival lymphocytes supports migration of activated memory T lymphocytes to diseased periodontal tissue
Oral Microbiol inmunol., 12 (1997), pp. 129-134
16.
C.M. Cobb,O. Singla,P. Feil,F. Theisen,R. Schultz
Comparison of NK-cell (Leu-7+ and Leu-11b+) populations in clinically healthy gingiva, chronic gingivitis and chronic adult periodontitis
J Periodontal Res., 24 (1989), pp. 1-7
17.
G. Seymour,J. Greenspan
The phenotypic characterization of lymphocyte subpopulations in established human periodontal disease
J Periodontal Res., 14 (1979), pp. 39-46
18.
J. Lindhe,B. Liljenberg,M. Listgarten
Some microbiological and histopathological features of periodontal disease in man
J Periodontol., 51 (1980), pp. 264-269
19.
H. Okada,T. Kida,H. Yamagami
Identification and distribution of immunocompetent cells in inflamed gingiva of human chronic periodontitis
Infection and Immunity., 41 (1983), pp. 365-374
20.
S. Passo,R.A. Reinhardt,L.M. DuBois,D. Cohen
Histological characteristics associated with suppurating periodontal pockets
J Periodontol., 59 (1988), pp. 731-740
21.
T. Modeer,G. Dahllof,E. Axio,K. Sundqvist
Subpopulations of lymphocytes in connective tissue from adolescents with periodontal disease
Acta Odontologica Scandinavia., 48 (1990), pp. 153-159
22.
T. Berglundh,B. Liljenberg,A. Tarkowski,J. Lindhe
Local and systemic TCR V gene expression in advanced periodontal disease
J Clin Periodontol., 25 (1998), pp. 125-133
23.
M. Taubman,T. Kawai
Involvement of T-lymphocytes in periodontal disease and in direct and indirect induction of bone resorption
Crit Rev Oral Biol Med., 12 (2001), pp. 125-135
24.
T. Berglundh,M. Donati
Aspects of adaptative host response in periodontitis
J Clin Periodontol., 32 (2005), pp. 87-107

 

Trabajo recibido el 27/02/2008.
Aprobado para su publicación el 01/07/2008.

CORRESPONDENCIA AUTOR
Nicolás Dutzan Muñoz
Olivos 943. Independencia. Santiago. Chile.
ndutzan@gmail.com

Copyright © 2008.
Sociedad de Periodoncia de Chile, Sociedad de Implantología Oral de Chile y Sociedad de Prótesis y Rehabilitación Oral de Chile.