Español    I    English              

Official body of scientific
expression of


Chilean Society of
Periodontology



Chilean Society of Oral
Oral Implantology



Chilean Society of Prosthetics and
Oral Rehabilitation

 


Chilean Society of
Odontopediatrics

 


Ibero-Panamerican Federation
of Periodontics

 


Ibero-Latin-American Association
of Oral Rehabilitation(AILARO)

Indexed in:

Scielo, ScienceDirect,
Latindex, Redalyc

Return to Index

Rev Clin Periodoncia Implantol Rehabil Oral. 2008;1:53-56
TRABAJO DE INVESTIGACIÓN [PDF]

Caracterización de Perfiles Proteicos y Peptídicos en Periodontitis Crónica

Characterization of Protein and Peptide Profiles in Chronic Periodontitis

Hernández M1,3, Obregón F1, Pozo P1, Barriga A2, Valenzuela MA2.

1Laboratorio de Biología Periodontal, Departamento de Odontología Conservadora, Facultad de Odontología, Universidad de Chile. Chile.
2Depto. de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile. Chile.
3Departamento de Patología, Facultad de Odontología, Universidad de Chile. Chile.

Resumen

La destrucción de los tejidos de soporte durante la periodontitis crónica está mediada por proteasas específicas. Producto de la actividad proteolítica se generarán cambios en la composición peptídica y proteica globales del fluido crevicular gingival (FCG). El objetivo de este estudio es evaluar la existencia de patrones de proteicos y peptídicos característicos en el FCG de pacientes con periodontitis crónica y sanos.

Materiales y métodos

Se seleccionaron 8 pacientes con periodontitis crónica moderada a severa y 5 sujetos controles y se obtuvieron muestras de FCG. Las muestras se eluyeron en 2 tampones, y se prepararon en 3 matrices distintas, y se obtuvieron los espectros peptídicos y proteicos mediante espectrometría de masas MALDI-TOF. Se procesaron 3 espectros de cada grupo para la obtención final de espectros diferenciales en enfermos.

Resultados

Se obtuvieron patrones proteicos y peptídicos reproducibles en el FCG. En pacientes con periodontitis crónica encontramos perfiles característicos representados por más de 100 proteínas que no fueron detectables en sujetos sanos.

Conclusiones

Demostramos la utilidad de la aplicación de la espectrometría de masas MALDI-TOF en el análisis de cambios cualitativos en la composición global del FCG y presentamos los perfiles proteicos y peptídicos característicos del FCG en periodontitis crónica y sujetos sanos.

Palabras claves: Periodontitis crónica, FCG, MALDI-TOF, perfiles proteicos.

Abstract

Inflammatory response mediates periodontal tissue destruction occurring in chronic periodontitis. As result, qualitative changes in global peptide and protein GCF composition will generate.

Aim

To evaluate existence of characteristic peptide and protein profiles in gingival crevicular fluid (GCF) from patients with chronic periodontitis and controls.

Methods

Patients with moderate to severe chronic periodontitis (n=8) and healthy controls (n=5) were selected and samples of GCF were obtained. Samples were eluted in 2 different buffers and prepared with 3 matrices to obtain peptide and protein spectra by MALDI-TOF mass spectrometry analysis. Subsequently, 3 spectra from each group were processed to obtain final differential spectra.

Results

Reproducible peptide and protein profiles were obtained from both stages in GCF. In disease samples we found characteristic patterns represented by more than 100 proteins which were undetectable in controls.

Conclusions

We demonstrate for the first time the usefulness of MALDI-TOF mass spectrometry in the analysis of qualitative global changes in GCF composition and we present protein and peptide characteristic GCF profiles associated with chronic periodontitis and healthy periodontium.

Key words: Chronic periodontitis, GCF, MALDI-TOF, protein profiles.

El Texto completo solo está disponible en PDF

 

Bibliografía

1.
L.N. Borrell,P.N. Papapanou
Analytical epidemiology of periodontitis
J Clin Periodontol, 32 (2005), pp. 132-158
2.
T. Kojima,E. Andersen,J.C. Sanchez,M.R. Wilkins,D.F. Hochstrasser,W.F. Pralong,G. Cimasoni
Human gingival crevicular fluid contains MRP8 (S100A8) and MRP14 (A100A9), two calcium binding proteins of the S100 family
J Dent Res, 79 (2000), pp. 740-747
3.
M.A. Curtis,J. Sterne,S.J. Price,G.S. Griffiths,S.K. Coulthurst,J.M. Wilton,N.W. Johnson
The protein composition of gingival crevicular fluid sampled from male adolescents with no destructive periodontitis: Baseline data of a longitudinal study
J Periodont Res, 25 (1990), pp. 6-16
4.
T. Ingman,T. Tervahartiala,Y. Ding,H. Tsesche,A. Haerian,D.F. Kinane,Y.T. Konttinen,T. Sorsa
Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients
J Clin Periodontol, 23 (1996), pp. 1127-1132
5.
C. López-Otín,C. Overall
Protease degradomics: a new challenge for proteomics
Nat Rev Mol Cell Biol, 3 (2002), pp. 509-519
6.
X. Puente,L. Sánchez,C. Overall,C. López-Otín
Human and Mouse proteases: a comparative genomic approach
Nat Rev Genet, 4 (2003), pp. 544-556
7.
A. Pardo,M. Selman
Matrix metalloproteinases in aberrant fibrotic tissue remodeling
Proc Am Thorac Soc, 3 (2006), pp. 383-388
8.
M.N. VanSaun,L.M. Matrisian
Matrix metalloproteinases and cellular motility in development and disease
Birth Defects Res (part C), 78 (2006), pp. 69-79
9.
C.M. Overall,R.A. Dean
Degradomics: Systems biology of the protease web. Pleiotropic roles of MMPs in cancer
Cancer Metastasis Rev, 25 (2006), pp. 69-75
10.
A.C. Newby
Matrix metalloproteinases regulate migration, proliferation, and death of vascular smooth muscle cells by degrading matrix and non-matrix substrates
Cardiovascular Res, 69 (2006), pp. 614-624
11.
C. Overall,C. McCulloch
Proteolytic host cell enzymes in gingival crevice fluid
Periodontolgy 2000, 31 (2003), pp. 77-104
12.
B.G. Loos,S. Tjoa
Host-derived diagnostic markers for periodontitis: do they exist in gingival crevice fluid?
Periodontol, 39 (2000 2005), pp. 53-72
13.
M. Hernández,M.A. Valenzuela,C. Lopez-Otin,J. Alvarez,J.M. Lopez,R. Vernal,J. Gamonal
Matrix metalloproteinase-13 is highly expressed in destructive periodontal disease activity
J Periodontol., 77 (2006), pp. 1863-1870
14.
M. Hernández,B. Martínez,J.M. Tejerina,M.A. Valenzuela,J. Gamonal
MMP-13 and TIMP-1 determinations in progressive chronic periodontitis
J Clin Periodontol., 34 (2007), pp. 729-735
15.
E. Adonogianaki,J. Mooney,D.F. Kinane
Detection of stable and active periodontitis sites by clinical assesment and gingival accute.phase protein levels
J Periodont Res, 31 (1996), pp. 135-143
16.
G.E. Salvi,N.P. Lang
Host response modulation in the management of periodontal diseases
J Clin Periodontol, 32 (2005), pp. 108-129
17.
R. Vernal,A. Chaparro,R. Graumann,J. Puente,M.A. Valenzuela,J. Gamonal
Levels of cytokine receptor activator of nuclear factor κB ligand in gingival crevicular fluid in untreated chronic periodontitis patients
J Periodontol, 75 (2004), pp. 1586-1591
18.
M. Mäkelä,E. Söderling,K. Paunio,J. Talonpoika,T. Hyyppä
Protein composition of crevicular fluid before and after treatment
Scand J Dent Res., 99 (1991), pp. 413-423
19.
F.T. Lundy,D.F. Orr,C. Shaw,P.J. Lamey,G.J. Linden
Detection of individual human neutrophil alpha-defensins (human neutrophil peptides 1, 2 and 3) in unfractionated gingival crevicular fluid -a MALDI-MS approach
Mol Immunol., 42 (2005), pp. 575-579
20.
E. Pisano,T. Cabras,C. Montaldo,V. Piras,R. Inzitari,C. Olmi,M. Castagnola,I. Messana
Peptides of human gingival crevicular fluid determined by HPLC-ESI-MS
Eur J Oral Sci., 113 (2005), pp. 462-468

 

Trabajo recibido el 31 marzo 2008, Aceptado
Aprobado para su publicación el 13 mayo 2008

Correspondencia autor:
Marcela Hernández Ríos
mhernandezrios@gmail.com

Copyright © 2008.
Sociedad de Periodoncia de Chile, Sociedad de Implantología Oral de Chile y Sociedad de Prótesis y Rehabilitación Oral de Chile.